Metabolic effects of high sucrose and saturated oil feeding on insulin resistance in Sprague-Dawley rats.

Abstract In this study, we explored the effects of long-term consumption of a high-sugar high-fat diet on glucose tolerance and insulin resistance in rats. Rats were fed with either standard rat chow diet (NC group) or high-sugar high-fat diet (HSHF group) for 16 weeks. The HSHF group showed significantly higher fasting insulin level than NC group. Following intraperitoneal glucose challenge, blood glucose and insulin levels in the NC and HSHF groups increased. However, the magnitude of the response in NC group was low compared to HSHF group. Insulin resistance was higher in HSHF group and insulin sensitivity decreased significantly (P < 0.05) in HSHF group in contrast to NC group. Low-density lipoprotein-cholesterol (LDL-C) and triglyceride (TG)/high-density lipoprotein-cholesterol (HDL-C) levels showed significant increase in HSHF group, while triglyceride and total cholesterol levels did not show any difference. The study demonstrated that feeding high-sugar high-fat diet to the experimental Sprague-Dawley rats for 16 weeks increased possibility of insulin resistance in them but did not turn them hyperglycemic or diabetic. Thus, they prove to be a suitable animal model to explore various aspects of insulin resistance.