沙蝇
病毒
生物
病毒学
显著性差异
精神科
兽医学
利什曼病
利什曼原虫
遗传学
医学
寄生虫寄主
万维网
计算机科学
内科学
作者
Xiuyan Xu,Jingxia Cheng,Shihong Fu,Qinyan Wang,Jing Wang,Xiaoyan Lu,Xiaodong Tian,Jianshu Cheng,Shuqing Ni,Ying He,Fan Li,Songtao Xu,Huanyu Wang,Bin Wang,Guodong Liang
出处
期刊:Vector-borne and Zoonotic Diseases
[Mary Ann Liebert]
日期:2021-04-01
卷期号:21 (4): 289-300
被引量:7
标识
DOI:10.1089/vbz.2020.2702
摘要
Background: Wuxiang virus was isolated from sandfly specimens collected in Wuxiang County, Shanxi Province, China in 2018, representing the first reported isolation of sandfly-borne virus from sandflies collected in a natural environment in China. The local sandfly density, seasonal fluctuations, and temporal and spatial distributions of the virus in Wuxiang County remain unclear. Materials and Methods: Four fixed sandfly collection sites were set up in Wuxiang County and sandfly specimens were collected continuously from June to August 2019. All sandfly specimens were subjected to viral isolation and molecular biological analysis. Results: The data on sandfly specimens collected in Wuxiang County from June to August 2019 showed a significant difference in the density of sandflies between June 26 and August 16 (p < 0.05). No statistical difference was found in sandfly density among collection sites (p > 0.05). A total of 33 virus isolates causing cytopathic effects in mammalian (BHK-21) cells were obtained from 7466 sandflies (91 pools) collected from June to August 2019. The results of molecular genetic evolution analysis of the nucleotide sequence of these isolates showed that the L and S genes (encoding NS and N proteins) of the 33 viruses isolated in 2019 are in the same evolutionary branch as the previously isolated Wuxiang virus. No significant difference was found in the virus isolation rate (the pool isolation rate of virus) among sandflies collected at different times from June to August (p > 0.05). The virus isolation rate of sandflies collected at different collection sites showed a statistically significant difference (p < 0.05). Conclusions: The results of this study suggest that the Wuxiang virus is a stable viral population in local sandflies. Strengthened research into Wuxiang virus infection of humans and animals and clarification of the public health hazards posed by Wuxiang virus to both humans and animals are urgently needed.
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