木犀草素
炎症体
脂多糖
化学
分子生物学
一氧化氮合酶
活性氧
半胱氨酸蛋白酶1
流式细胞术
污渍
巨噬细胞极化
巨噬细胞
活力测定
一氧化氮
细胞凋亡
生物化学
受体
生物
体外
免疫学
酶
类黄酮
有机化学
基因
抗氧化剂
作者
Bu-Chun Zhang,Zhi Li,Xu Wu,Chu-Han Xiang,Yanfeng Ma
出处
期刊:PubMed
日期:2018-01-01
卷期号:10 (1): 265-273
被引量:32
摘要
Pure plant extract luteolin has been demonstrated to possess numerous biological effects. However, the specific effect of luteolin on macrophage polarization and NOD-like receptor protein 3 (NLRP3) inflammasome activation has not been documented. In this study, Cultured RAW264.7 cells were treated with or without luteolin in the presence or absence of LPS. Subsequently, cell viability was tested by CCK-8 assay. Total reactive oxygen species (ROS) were measured by flow cytometry. NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), caspase-1, inducible nitric oxide synthase (iNOS) and Arginase (Arg-1) protein expression was detected using western blotting. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect the level of TNF-α, IL-18, and Interleukin-1β (IL-1β). Increased production of ROS and expression of NLRP3, ASC, caspase-1, IL-18 and IL-1β proteins were observed in RAW264.7 cells incubated with LPS and were effectively inhibited by 2 μM luteolin. Furthermore, 2 μM luteolin pretreatment enhanced the expression of M2 macrophage markers (Arg-1 and IL-10), and decreased the expression of markers associated with M1 macrophage polarization (TNF-α, IL-6 and iNOS). These results indicated that low-dose luteolin inhibits NLRP3 inflammasomes activation and promotes macrophage polarization toward an M2 phenotype, which provides new evidence for the anti-inflammation activity of luteolin.
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