This work investigates the design and the operation of microfluidic membrane devices aimed atthe real-time separation of blood plasma. The goal is to enhance separation accuracy and response time forpoint-of-care diagnostic devices. A hybrid approach that included computational modeling and experimentalvalidation was used. Results indicate higher plasma purity and recovery efficiency compared to conventionalcentrifugation techniques. The study demonstrates the promise of microfluidic devices in the field of medicaldiagnostic devices, offering an infrastructure for real-time, automated, and low-impact plasma extraction