化学
胶体金
表面等离子共振
纳米颗粒
灵敏度(控制系统)
粒子(生态学)
分析化学(期刊)
纳米技术
体积流量
检出限
色谱法
材料科学
海洋学
物理
量子力学
电子工程
地质学
工程类
作者
Shaobin Zhu,Lingling Yang,Yao Long,Min Gao,Tianxun Huang,Wei Hang,Xiaomei Yan
摘要
Employing single nanoparticle detection with a laboratory-built high-sensitivity flow cytometer, we developed a simple and versatile platform that is capable of detecting the surface plasmon resonance scattering of gold nanoparticles (GNPs) as small as 24 nm, differentiating GNPs of different sizes, and providing accurate quantification of GNPs. Low-concentration samples (fM to pM) in small volumes (μL) can be measured in minutes with an analysis rate of up to 100−200 GNPs per second. Among these features, absolute quantification provides a distinct advantage because it does not require standard samples.
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