常染色质
EZH2型
组蛋白H3
组蛋白甲基化
组蛋白
组蛋白甲基转移酶
生物
染色质
生物化学
甲基化
遗传学
分子生物学
基因
异染色质
DNA甲基化
基因表达
作者
Helena Santos‐Rosa,Robert Schneider,Andrew J. Bannister,Julia A Sherriff,B Bernstein,N. C. Tolga Emre,Stuart L. Schreiber,Jane Mellor,Tony Kouzarides
出处
期刊:Nature
[Springer Nature]
日期:2002-09-01
卷期号:419 (6905): 407-411
被引量:1923
摘要
Lysine methylation of histones in vivo occurs in three states: mono-, di- and tri-methyl. Histone H3 has been found to be di-methylated at lysine 4 (K4) in active euchromatic regions but not in silent heterochromatic sites. Here we show that the Saccharomyces cerevisiae Set1 protein can catalyse di- and tri-methylation of K4 and stimulate the activity of many genes. Using antibodies that discriminate between the di- and tri-methylated state of K4 we show that di-methylation occurs at both inactive and active euchromatic genes, whereas tri-methylation is present exclusively at active genes. It is therefore the presence of a tri-methylated K4 that defines an active state of gene expression. These findings establish the concept of methyl status as a determinant for gene activity and thus extend considerably the complexity of histone modifications.
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