Synthesis and Characterization of a Cascaded Chromogenic Enzyme Substrate

A novel galactoside derivative (1) incorporatingp-nitroaniline (p-NA) as chromophore has been synthesized and used as a cascaded chromogenic enzyme substrate for the detection of β-D-galactosidase, which was characterized using UV,1H NMR,13C NMR and MS spectra. A plausible mechanism for the release ofp-NPA from the enzyme substrate 1 was proposed.