前交叉韧带
肌腱
细胞外基质
脚手架
生物医学工程
材料科学
基质(化学分析)
体内
骨愈合
X射线显微断层摄影术
组织工程
化学
解剖
医学
复合材料
生物
放射科
生物技术
生物化学
作者
Qingsong Jiang,Liren Wang,Zhanhong Liu,Jinlei Su,Yajun Tang,Peijie Tan,Xiangdong Zhu,Kai Zhang,Xing Ma,Jia Jiang,Jinzhong Zhao,Hai Lin,Xingdong Zhang
标识
DOI:10.1016/j.bioactmat.2022.05.003
摘要
Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence. In this study, an injectable hydroxyapatite/type I collagen (HAp/Col Ⅰ) paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface. Physicochemical characterization demonstrated that the HAp/Col Ⅰ paste was injectable, uniform and stable. The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression. The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL. The average width of the tunnel, total bone volume, bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group. The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL's maximal load at 24 weeks. According to histological examination, Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group. The injectable HAp/Col Ⅰ paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation, further osteogenic expression and extracellular matrix deposition, and in vivo structural and functional regeneration of the tendon-bone interface.
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