Chemical and Genetic Probes of the Active Site of D ‐Ribulose‐1,5‐Bisphosphate Carboxylase/Oxygenase: A Retrospective Based on the Three‐Dimensional Structure

This chapter contains sections titled: Introduction Reaction Pathway Crystal Structures Active Site Characterization with General Reagents Active Site Characterization with Affinity Labels Chemical Properties of the Two Active-Site Lysines Identified with Affinity Labels and Their Inter-Residue Distance General Considerations of Site-Directed Mutagenesis and Characterization of Rubisco Mutants Chemical Rescue Site-Directed Mutagenesis Guided by Chemical Modification and Comparative Sequences Structure-Guided Mutagenesis Carboxylase/Oxygenase Specificity Concluding Remarks