脂肪酶
生物柴油生产
色谱法
化学
酯交换
大小排阻色谱法
甘油三酯酶
假单胞菌
生物柴油
生物化学
细菌
生物
酶
催化作用
遗传学
作者
Khushboo Khosla,Rashmi Rathour,Raj Maurya,Neha Maheshwari,Edgard Gnansounou,Christian Larroche,Indu Shekhar Thakur
标识
DOI:10.1016/j.biortech.2017.08.194
摘要
An extracellular lipase was purified and characterized from psychrotolerant bacterium Pseudomonas sp. ISTPL3 isolated from Pangong lake. Lipase was purified by sequential methods of ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange chromatography and Sephadex G-100 gel filtration chromatography, resulting in a purification fold of 6.53 and yield of 5.45%. The molecular weight was approximately 31kDa. The purified lipase was used for transesterification of lipids produced by oleaginous chemolithotrophic bacterium Serratia sp. ISTD04 for production of biodiesel. Upon biochemical characterization, lipase was found to be alkalophilc, thermostable, active in organic polar solvents and sensitive to detergents. Further, lipase was immobilized on activated biochar to assess its transesterification efficiency during biodiesel production. Immobilized lipase gave the highest yield of fatty acid methyl esters (FAMEs) (92.23%)>unimmobilized lipase>NaOH. The immobilized lipase was assessed for its reusability and retained 75.11% of its activity after 3 cycles of biodiesel production.
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