木二糖
化学
木聚糖酶
水解
酶水解
木聚糖
木糖
热液循环
生物化学
食品科学
色谱法
酶
化学工程
发酵
工程类
作者
Yan Su,Lingyan Fang,Peng Wang,Chenhuan Lai,Caoxing Huang,Zhe Ling,Shaolong Sun,Qiang Yong
标识
DOI:10.1016/j.biortech.2021.125955
摘要
A promising approach for production of value-added xylooligosaccharides (XOS) from poplar was developed by combining hydrothermal pretreatment and endo-xylanase post-hydrolysis. Results showed that the 35.4% XOS (DP 2-6) and 17.6% low DP xylans (DP > 6) were obtained at the identified optimal condition (170 °C, 50 min) for hydrothermal pretreatment. Structural features of low DP xylans generated during the hydrothermal pretreatment were examined, revealing that low DP xylans are mainly comprised of 4-O-methylglucuronic xylan and are involved in lignin carbohydrate complexes. Moreover, higher pretreatment intensity promoted the cleavage of side-chain substituents including arabinose and glucuronic acid groups. The subsequent endo-xylanase hydrolysis of the pretreatment liquor hydrolyzed low DP xylans, contributing to a significant improvement in xylobiose and xylotriose proportions. This combined strategy resulted in a XOS with conversion yield of 44.6% containing 78.7% xylobiose and xylotriose starting from the initial xylan in raw poplar.
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