BODIPYs as Fluorogenic Probes for Live-cell Imaging of Lipid Droplets: Photophysical and Computational Studies

Introduction: Lipid droplets (LDs) serve as primary storage sites for neutral lipids within cells and are crucial for lipid metabolism. Disorders affecting LDs can contribute to the pathogenesis of common metabolic diseases such as obesity and cancer, highlighting the importance of comprehend-ing LD biology in health and disease contexts. Background: Fluorescence assays are commonly used for the detection and quantification of lipids in bio-logical samples or lipid-rich environments. In this study, BODIPYs were synthesized and analyzed for structural confirmation. These compounds were subsequently evaluated for photophysical, electro-chemical (cyclic voltammetry) and theoretical analysis, followed by live-cell imaging studies to con-firm their affinity for intracellular lipid droplets. Results: BODIPYs have been identified as fluorogenic probes for live-cell imaging studies and found to serve as efficient and selective fluorescent substances for intracellular lipid droplets. Conclusion: These BODIPYs, especially 2b, are valuable addition to the expanding toolkit for intra-cellular diagnostics, offering versatility and reliability across various cellular imaging applications.