萃取(化学)
化学
纤维素酶
色谱法
花椒
尿素
响应面法
甲醇
酶
植物
有机化学
生物
作者
Si-wei Rao,Feng‐Xiang Zhang,Yupeng Duan,Z Chen,Dong Liang,Wei Shi
标识
DOI:10.1016/j.microc.2024.109964
摘要
In this work, an enzymatic digestion urea-based solvent system was developed to extract alkaloids and flavonoids in Zanthoxylum nitidum (Roxb.) DC (ZN). A total of 50 DESs were prepared and their extraction rate were screened, and the enzyme conditions affecting the rate were also evaluated. As a result, it was found that the Ur/Pa based cellulase system owned the best extraction efficiency in ZN with the rate of 34.56 ± 1.09 mg/g, which was higher than the methanol. Optimized using the one factor experiments and response surface methodology (RSM), the best condition was 27 % water content, 35 min extraction time, 39℃ extraction temperature, 25:1 solid/liquid ratio, 1.3 mg/mL enzyme concentration. The scanning electron microscopy (SEM) results showed that the Ur/Pa based cellulase system entirely destroys the cell wall of ZN, with excellent extraction rate. This study developed a new design of DESs in extraction of alkaloids and flavonoids in ZN.
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