生物
生殖系
拟南芥
转座因子
遗传学
表观遗传学
阿尔戈瑙特
表观基因组
Piwi相互作用RNA
拟南芥
体细胞
小RNA
细胞生物学
RNA干扰
核糖核酸
DNA甲基化
基因组
基因表达
突变体
基因
作者
Gabriele Bradamante,Vu Nguyen,Marco Incarbone,Zohar Meir,Heinrich Bente,Mattia Donà,Nicole Lettner,Ortrun Mittelsten Scheid,Ruben Gutzat
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2023-12-07
卷期号:36 (4): 863-880
标识
DOI:10.1093/plcell/koad295
摘要
Abstract In sexually propagating organisms, genetic, and epigenetic mutations are evolutionarily relevant only if they occur in the germline and are hence transmitted to the next generation. In contrast to most animals, plants are considered to lack an early segregating germline, implying that somatic cells can contribute genetic information to progeny. Here we demonstrate that 2 ARGONAUTE proteins, AGO5 and AGO9, mark cells associated with sexual reproduction in Arabidopsis (Arabidopsis thaliana) throughout development. Both AGOs are loaded with dynamically changing small RNA populations derived from highly methylated, pericentromeric, long transposons. Sequencing of single stem cell nuclei revealed that many of these transposons are co-expressed within an AGO5/9 expression domain in the shoot apical meristem (SAM). Co-occurrence of transposon expression and specific ARGONAUTE (AGO) expression in the SAM is reminiscent of germline features in animals and supports the existence of an early segregating germline in plants. Our results open the path to investigating transposon biology and epigenome dynamics at cellular resolution in the SAM stem cell niche.
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