衰老
茉莉酸
水杨酸
生物
拟南芥
转录因子
拟南芥
细胞生物学
WRKY蛋白质结构域
基因表达
苯丙氨酸解氨酶
基因
生物化学
突变体
氨基酸
苯丙氨酸
作者
Shuangcheng He,Fang Zhi,Yuanchang Min,Ruixin Ma,Ankang Ge,Shixiang Wang,Jianjun Wang,Zijin Liu,Yuan Guo,Mingxun Chen
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2022-12-21
卷期号:192 (1): 488-503
被引量:6
标识
DOI:10.1093/plphys/kiac589
摘要
Leaf senescence is the final stage of leaf development and is affected by various exogenous and endogenous factors. Transcriptional regulation is essential for leaf senescence, however, the underlying molecular mechanisms remain largely unclear. In this study, we report that the transcription factor MYB59, which was predominantly expressed in early senescent rosette leaves, negatively regulates leaf senescence in Arabidopsis (Arabidopsis thaliana). RNA sequencing revealed a large number of differentially expressed genes involved in several senescence-related biological processes in myb59-1 rosette leaves. Chromatin immunoprecipitation and transient dual-luciferase reporter assays demonstrated that MYB59 directly repressed the expression of SENESCENCE ASSOCIATED GENE 18 and indirectly inhibited the expression of several other senescence-associated genes to delay leaf senescence. Moreover, MYB59 was induced by salicylic acid (SA) and jasmonic acid (JA). MYB59 inhibited SA production by directly repressing the expression of ISOCHORISMATE SYNTHASE 1 and PHENYLALANINE AMMONIA-LYASE 2 and restrained JA biosynthesis by directly suppressing the expression of LIPOXYGENASE 2, thus forming two negative feedback regulatory loops with SA and JA and ultimately delaying leaf senescence. These results help us understand the novel function of MYB59 and provide insights into the regulatory network controlling leaf senescence in Arabidopsis.
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