Differences caused by blood and secretion samples for the detection of Klebsiella pneumoniae using metagenomic next-generation sequencing: A comparative analysis

•Blood sampling is an efficient method for utilizing mNGS to detect pathogens such as Klebsiella pneumoniae. •The quantity of Klebsiella pneumoniae reads identified in blood accounted for a small fraction of those in secretions. •The number of microorganisms identified in blood was significantly fewer than those in secretions. •The microorganisms detected in both blood and secretions constituted the largest fraction among those in blood.