脂磷壁酸
地氯酸
枯草芽孢杆菌
单核细胞增生李斯特菌
微生物学
细菌
免疫印迹
细菌细胞结构
单克隆抗体
单元格信封
化学
斑点印迹
金黄色葡萄球菌
生物
生物化学
抗体
大肠杆菌
DNA
遗传学
免疫学
基因
作者
Charlotte Millership,Angelika Gründling
出处
期刊:Methods in molecular biology
日期:2023-10-11
卷期号:: 95-106
标识
DOI:10.1007/978-1-0716-3491-2_8
摘要
Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, and Listeria monocytogenes. The polymer is linked by a lipid anchor to the outer leaflet of the bacterial membrane and in some bacteria can also be shed and detected in the culture supernatant. Here, we describe a simple and rapid western blot method for the detection of Type I LTA in bacterial cell extracts and culture supernatant fractions using a polyglycerol phosphate specific monoclonal LTA antibody.
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