Synthesis of Ag nanoparticles for selective dual detection of glutathione and dopamine using N, N-dimethyl-p-phenylenediamine mediated colorimetric probe

We report a simple and easy method to synthesize Ag nanoparticles (Ag NPs) and demonstrate its potential for the detection of glutathione (GSH) and dopamine (DA) via colorimetric assay. The Ag NPs were found to be monodispersed and spherical with a size of 5 ± 2 nm. The X-ray diffraction (XRD) and high resolution transmission electron microscopy (HR-TEM) investigations revealed the formation of crystalline Ag NPs. The colour of N, N-dimethyl-p-phenylenediamine assay changed from dark pink to colourless when the concentration of GSH was increased from 1 to 40 μM. Notably, the suspension colour changed from dark pink to blue when a similar set of experiments were performed with DA. The UV/Visible and interference experiments of Ag NPs exhibited excellent sensitivity and selectivity against both GSH and DA even after the addition of 40 μM of different interference biomolecules. The calculated limit of detection (LOD) was 141 and 245 nM for GSH and DA, respectively. The real-time analysis with serum samples showed satisfactory recovery percentages of >95 and 80-90% for GSH and DA, respectively. Hence, the Ag NPs reported here have huge potential to serve as a sensitive and selective colorimetric sensor for the detection of GSH and DA for diverse applications ranging from catalysis to cancer therapy and theranostics.