荧光
肺炎
荧光寿命成像显微镜
近红外光谱
红外线的
材料科学
检出限
病变
医学
光学
化学
病理
内科学
物理
色谱法
作者
Erwang Song,Youbo Lai,Huixu Lu,Yonghe Tang,Lintao Zeng,Weiying Lin
摘要
The mortality rate of pneumonia increases significantly with the prolongation of illness. In the pursuit of a tool to accurately diagnose pneumonia in its early stages, we designed and synthesized a two-photon near-infrared fluorescent probe (DCQN) to identify increased concentrations of the inflammation marker SO2. The probe was found to specifically react with SO2 by undergoing a 1,4-addition reaction to generate near-infrared fluorescence with good sensitivity (6 s), a large Stokes shift (110 nm) and low detection limit (1.49 nM). DCQN has near-infrared emission as well as good two-photon performance, which can image exogenous and endogenous SO2 in cells and avoid interference from background fluorescence from cells. Furthermore, this study achieved accurate imaging of a pneumonia lesion site in deep tissues to provide a tool for the fluorescence diagnostic imaging of pneumonia in situ.
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