Engineered Pine Endophytic Fungus Expressing Double-Stranded RNA Targeting Lethal Genes to Control the Plant-Parasitic Nematode Bursaphelenchus xylophilus

was significantly suppressed. Notably, nematode populations that consumed fungi expressing dsL1177 and dsAK1 showed substantial declines over time. These findings provide novel insights and a practical foundation for employing endophytic fungi-expressed dsRNA in sustainable pest management strategies.