CD14型
体外
免疫系统
免疫耐受
免疫学
细胞生物学
树突状细胞
抗原提呈细胞
生物
周边公差
T细胞
生物化学
作者
Catharien Hilkens,Julie Diboll,Fiona Cooke,Amy E. Anderson
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 477-492
被引量:2
标识
DOI:10.1007/978-1-0716-3135-5_31
摘要
Human monocyte-derived dendritic cells (moDC) are commonly used as a research tool to investigate interactions between antigen-presenting cells and T cells. Generation of these cells involves the isolation of CD14 positive monocytes from peripheral blood and their in vitro differentiation into immature moDC by the cytokines GM-CSF and IL-4. Their functional characteristics can then be manipulated by maturing these cells with a cocktail of agents, which can be tailored to induce either immune activating or tolerogenic properties. Here, we describe a protocol for the generation of moDC with stable tolerogenic function, referred to as tolerogenic dendritic cells. These cells have been developed as an immunotherapeutic tool for the treatment of autoimmune disease but have also proven useful to dissect mechanisms of T cell tolerance induction in vitro.
科研通智能强力驱动
Strongly Powered by AbleSci AI