Optimization of wheat high molecular weight glutenin subunit(HMW-GS) is an important approach for genetic improvement of wheat quality and confirmation of quality effects of HMW-GS and their pyramiding manner is the key foundation.In the current paper,quality effects of 8 HMW-GS and 13 pyramiding manner were studied using 33 wheat cultivars as the experimental materials.The contributions of variation in HMW-GS to the quality traits were determined as: in 1A chromosome,1 ≈ N(absence) to the effects on gluten quantity and strength;in 1B chromosome,14+15 7+87+9 to the positive effects on gluten quantity and 7+814+157+9 to gluten strength;in 1D chromosome,2+125+104+12 to the positive effects on gluten quantity and 5+102+12 4+12 to gluten strength.Effects of different excellent HMW-GS on quality traits were accumulative.Optimized pyramiding manner of high gluten quantity type and strong gluten type varieties were put forward according to the results described above.The subunit effects on quality traits was further affirmed by correspondence analysis of cultivar type and HMW-GS gene pyramiding manner.