An Indirect-enzyme linked Immunosorbent Assay for Detection of Antibody to Porcine Reproductive and Respiratory Syndrome Virus

In this study,differential centrifugation and discontinuous sucrose density gradient centrifugation were adopted to concentrate and purify porcine reproductive respiratory and syndrome virus(PRRSV),The purified PRRSV which is treated with Trichlor_trifluorethan regard as ELISA_antigen.The indirect ELISA was sensitive and specific,and it was superior to both IPMA and IFA in detecting antibodies in the sensitive.