Experimental study on immunologic expression of TNF-α and IL-6 in lung tissues of rats with insufficiency of the lung-qi and lung-yang

Objective To observe the change of immunologic expression of tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6) in lung tissues of model rats with insufficiency of the lung-qi and lung-yang.Methods Health wistar rats were divided into control group,insufficiency syndrome of the lung-qi and lung-yang group.The models of insufficiency of the lung-qi were induced by the smoke of saw power and cigarettes for consecutive 7 weeks.The model rats of insufficiency of the lung-yang were placed into the fridge for 2 hours per time for 7 weeks after smoking 30 minutes,every day was 2 times.At the end of 7 weeks,the lung tissue was taken for observing pathologic picture and detecting the immunologic expression of TNF-α and IL-6 by SABC technique of immunohistochemistry.Results Compared with control group,the total positive area and average optical density of TNF-α and IL-6 were obviously increased in insufficiency syndrome of the lung-qi and lung-yang group(P0.05 or P0.01).Compared with insufficiency syndrome of the lung-qi group,the total positive area of TNF-α was obviously increased(P0.05).Conclusion TNF-α and IL-6 were involved in the pathological process of insufficiency of the lung-qi and lung-yang,and may promote the process from insufficiency of the lung-qi to insufficiency of the lung-yang.