The use of random amplification of polymorphous bacterial DNA for the typing of bacteria in the genus Citrobacter

To obtain the profiles of randomly amplified DNA, isolated from bacteria of the genus Citrobacter, the method of polymerase chain reaction was used. Nine oligonucleotides were evaluated for the possibility of their use as primers for the amplification of random polymorphous sequences of DNA; of these, 2 oligonucleotides which generated profiles, sufficiently reproducible and typical for different C. freundii and C. diversus strains, were selected. The possibility of using the above oligonucleotides in pair for amplification of species-specific fragments of polymorphous bacterial DNA for typing was shown.