To determine population structure and distribution of Fusarium species causing head blight of wheat,60 wheat samples showing symptoms of Fusarium head blight from 15 areas 8 cities and counties were collectted in Northeast of China. A total of 118 Fusarium isolates were identified based on morphological characters and modern molecular systematics method. The results showed that 118 Fusarium isolates belong to 7 species,Fusarium graminearum was the most prevalent species,representing 64. 41% of the total isolates,followed by Fusarium fujikuroi,representing 18. 64%,Fusarium avenaceum,Fusarium oxysporum,Fusarium equiseti,Fusarium acuminatum and Fusarium verticillioides representing 5. 08%,3. 39%,3. 39%,3. 39% and 1. 70%. At the same time,their pathogenicity were determined in stem of adult-stage maize,the results showed that Fusarium graminearum isolated from wheat can infect maize,but the pathogenicity of Fusarium graminearum causing maize stalk rot were significantly greater than the Fusarium graminearum causing wheat head scab.