Determination of Four Kinds of Alkaloids in Coptis Chinensis Purified by Macroporous Absorption Resin

Objective: To establish a method for determining the contents of four kinds of alkaloids in Coptis chinensis purified by macroporous absorption resin by Rp- HPLC. Methods: The macroporous absorption resin X- 5 was used to separate and purify the total alkaloids from Coptis chinensis. The HPLC method was used to determine the contents of epiberberine,coptisine,palmatine and berberine. Results: The average contents of epiberberine,coptisine,palmatine and berberine in Coptis chinensis with macroporous absorption resin were 1. 45%,2. 62%,7. 03%,17. 95% and the average contents without macroporous absorption resin were 0. 67%,1. 05%,1. 86% and 8. 54%. Conclusion: The alkaloids in Coptis chinensis can be effectively separated and purified with the macroporous absorption resin X- 5.