Deter mination and significance of excitation a mino acids transmitters in blood and cerebrospinal fluid of patients with cerebral infarction

Objective Observe the dynamic changes of EAA in patients with cerebral infarction and the relation of EAA levels to infarct size and intensity of neurological deficit, the EAA in blood and cerebrospinal fluid(CSF) were detected to investigation the effect of EAA to the time course of in Ischemic stroke. Methods High performance liquid chromatography(HPLC) was use to detected the levels of Glu and Asp in blood and CSF. The samples were taken from 33 cases of patients with cerebral infarction during 7 hours～10 days after the ictus and 10 healthy persons were detected as the normal control. Results Concentrations of Glu and Asp began to increase significantly in 24 hours, and in the 3rd day reached the peak. It began to decrease after the 5th day and 7 days after infarction it returned to normal level. The concentration of Glu and Asp in blood correlated positively to that in CSF after infarction.The levels of Glu significantly correlated with the diameter of the infarction site,stage and neurological functional scales. Conclusion The Glu and Asp played the important roles in the pathologic process of cerebral infarction, the detection of Glu and Asp were helpful to the study of basis and clinic of cerebral infarction.