Expression of mitochondrial prohibitin in paclitaxol-resistant CCRF-CEM cells

Objective: To elucidate the role of mitochondrial prohibitin in multi-drug resistant CCRF-CEM cells by determination of expression status of the prohibitin.Methods: The total mitochondrial proteins from CCRF-CEM cells or paclitaxol-resistant CCRF-CEM cells were extracted. The isolated proteins were separated by two-dimensional electrophoresis and then analyzed using ImageMasterTM 2D Platinum software.The protein spots on the gel were analyzed by MS and identified by comparison to SWISS-PROT database and NCBInr database.Results: One of the two mitochondrial prohibitins was over-expressed in CCRF-CEM/paclitaxol by 68.6%(per protein volume expressed) compared with that in CCRF-CEM cells.Conclusion: The mitochondrial prohibitin may reduce multidrug resistances against the paclitaxol-induced CCRF-CEM cellular apoptosis by regulation of the mitochondria activities.