Microscopic Imaging Technology of Second Harmonic Generation

As a new technology of nonlinear microscopic imaging,second harmonic generation(SHG) have been widely used in all fields of medicine and biology.In this paper,we analyzed the principle of SHG,introduced the setup and development of SHG technology.Then we made a comparison between SHG and two-photon excited fluorescence(TPEF).Some applications in biomedicine were also given.Lastly we predicted the further development and application of SHG.