Effects of acoustic parameters on nanodroplet vaporization

Phase shift nanodroplets offer a number of advantages over ordinary microbubbles due to their enhanced stability and smaller size distribution. These nanodroplets undergo a phase transition from liquid to highly echogenic gaseous state when activated by sufficient acoustic energy through a process termed acoustic droplet vaporization (ADV). In this study, we synthesized lipid-coated perfluoropentane (PFP) filled nanodroplets via sonication method. We investigated the ADV threshold of these nanodroplets as a function of acoustic parameters such as excitation pressure, frequency, pulse length, and pulse repetition period (PRP). Our results indicate that ADV threshold varies significantly with PRP; while at PRP of 10 ms, the ADV threshold was found to be 3.6 MPa (pk-pk), for PRP of 1 ms, 100 μs, and 500 μs, ADV was not observed even at 10 MPa. At ADV, fundamental and odd harmonics were found to be significantly higher than the background noise. The acoustic response of ordinary perfluorobutane (PFB) filled microbubbles with the same lipid composition is compared to that of PFP nanodroplets when both were excited with the same excitation pressures (450 kPa to 10 MPa). Above ADV threshold both showed a similar response.