A Study on Thioacetamide-induced Rat Model of Liver Cirrhosis and the Effect of Endotoxin in the Development of Cirrhosis

Objective: To establish the stable and efficient rat hepatic fibrosis model induced by Thioacetamide(TAA),study the best does of TAA and to observe the pathology and function of liver,changes in alanineaminotransferase(ALT) and endotoxin.Methods: 30 SD male rats were selected and randomly divided into 2 groups,the control group(15)and the model group(15).The control group was treated with only water and the model group was treated with 0.03 % TAA(first six weeks) and 0.04 % TAA(latter six weeks).After twelve weeks,all rats were executed and made into hepaticsmears.Results: Mortality in the model group was 4.17 % and the rate of hepatic fibrosis was 95.83 %.Through the Light microscope,the normal lobular structure was found disappeared and the false lobules formed.The content levels of serum ALT and serum endotoxin in the plasma of model group were higher than those in the control group.Conclusion: 0.03 % TAA-induced cirrhosis showed a low mortality and high success rate,and that endotoxin can accelerate liver fibrosis and the formation of cirrhosis.