[Effect of UBE2C overexpression on the proliferation of 293T cell line].

To establish a 293T cell line with stable expression of UBE2C and to investigate the effect of UBE2C overexpression on the proliferation of 293T cells.Recombinant plasmid pcDNA3.1(-)/UBE2C successfully constructed in previous time was transfected into 293T cells by lipofectamine 2000. After screened with G418 for 4 weeks, Western blot was used to detect the protein level of UBE2C and MTT assay to detect the proliferation of 293T cells.Compared with pcDNA3.1(-) transfected group, Western blot analysis demonstrated that the level of UBE2C was significantly increased in pcDNA3.1(-)/UBE2C transfected group (P < 0.05). MTT assay showed that 293T cells overexpressing UBE2C proliferated faster than control 293T cells.A 293T cell line with UBE2C stable expression is successfully established and UBE2C promoted the proliferation of 293T cells.