Role of the Rho/Rho-kinase pathway in the mechanism of hypoxic pulmonary vasoconstriction

Objective To observe the signaling pathway of 15-hydroxyeicosatetraenoicacid(15-HETE) induced pulmonary artery constriction of rats,and clarify the possible mechanism of 15-HETE induced hypoxic pulmonary vasoconstriction.Methods Pulmonary arterial rings were used to assess the role of 15-HETE in pulmonary artery tension from hypoxic and normoxic rats,furthermore,the inhibitor of Rho-kinase signaling pathway was employed to determine the role of Rho/Rho-kinase signaling pathway in 15-HETE induced pulmonary vasoconstriction.Results 15-HETE constricted the pulmonary artery rings of both hypoxic and nomorxic rats;and Y-27632 reduced 15-HETE vasoconstriction effect significantly(P0.05).Conclusion 15-HETE constricts the pulmonary arteries of rats in chronic hypoxia via Rho/Rho-kinase signaling pathway.