Dentification on cultured bacteria of Tianchi water by 16S rRNA analysis

To study the type and diversity of bacteria from Tianchi water,isolated 25 bacterial strains with NA,1/10NA,R2A and 1/10R2A as culture medium from Tianchi water samples,16S rRNA gene sequences combined with phylogenetic analysis and morphological observation were used to separate and identify the bacteria from Tianchi water.Finally,nine kinds of bacterial strains were obtained and the phylogenetic tree was established.The results showed that 9 bacteria isolated from Tianchi water associated with Bacillus and Anoxybacillus genus in phylogenetics closely,among which Bacillus genus was the dominant species.