蛋白质组
相扑蛋白
泛素连接酶
蛋白质组学
人类蛋白质组计划
计算生物学
DNA连接酶
生物
蛋白质微阵列
泛素蛋白连接酶类
鉴定(生物学)
泛素
微阵列
生物信息学
遗传学
基因
基因表达
植物
作者
Eric Cox,Ijeoma Uzoma,Catherine M. Guzzo,Jun Seop Jeong,Michael J. Matunis,Seth Blackshaw,Heng Zhu
出处
期刊:Methods of Molecular Biology
日期:2015-01-01
卷期号:: 455-463
被引量:10
标识
DOI:10.1007/978-1-4939-2550-6_32
摘要
The functional protein microarray is a powerful and versatile systems biology and proteomics tool that allows the rapid activity profiling of thousands of proteins in parallel. We have recently developed a human proteome array, the HuProt array, which includes ~80 % of all the full-length proteins of the human proteome. In one recent application of the HuProt array, we identified numerous SUMO E3 ligase-dependent SUMOylation substrates. For many SUMO E3 ligases, only a small number of substrates have been identified and the target specificities of these ligases therefore remain poorly defined. In this protocol, we outline a method we developed using the HuProt array to screen the human proteome to identify novel SUMO E3 ligase substrates recognized by specific E3 ligases.
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