金黄色葡萄球菌
化学
毛细管电泳
纳米颗粒
色谱法
聚合酶链反应
吸附
细菌
DNA提取
萃取(化学)
磁性纳米粒子
微生物学
纳米技术
生物化学
生物
有机化学
基因
材料科学
遗传学
作者
Xiang Gao,Xin Yao,Zi‐Tao Zhong,Jia Li
出处
期刊:Talanta
[Elsevier]
日期:2018-08-01
卷期号:186: 147-153
被引量:28
标识
DOI:10.1016/j.talanta.2018.04.046
摘要
Pathogens cause significant morbidity and mortality to humans. Thus, development of fast and reliable methods for detection and identification of pathogens is urgently needed to increase protection level of public health and ensure the safety of consumers. Herein, a rapid and sensitive method has been developed for Staphylococcus aureus (S. aureus) detection based on the dual role of polydopamine modified magnetic nanoparticles ([email protected]3O4 NPs) combined with polymerase chain reaction (PCR) and capillary electrophoresis (CE). The core-shell type structure [email protected]3O4 NPs were prepared, which are spherical, about 152 ± 20 nm in diameter and the PDA shell is about 17.5 ± 1.6 nm. [email protected]3O4 NPs play a dual role including efficient capture of bacteria and extraction of DNA. In the pH range of 3.0–7.0, the capture efficiency of S. aureus by [email protected]3O4 NPs was more than 95% in 5 min. The adsorption capacity of the [email protected]3O4 NPs for S. aureus is 1.2 × 108 cfu mg−1. The efficient capture and concentration of bacteria from large volumes of samples by [email protected]3O4 NPs avoids the time-consuming culture-enrichment prior to PCR. Interestingly, [email protected]3O4 NPs were also found to be efficient adsorbents for extraction of genomic DNA from pathogens based on the electrostatic interaction. The process can be finished in 25 min. The [email protected]3O4 NPs based solid phase extraction combined with PCR and CE allows for detecting the order of 102 cfu mL−1 S. aureus in tap water and orange juice samples. The whole process takes < 5.5 h. The developed method would provide a promising platform for rapid and sensitive detection of pathogens.
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