Amphiphilic guanidine-based lipids enhance mRNA delivery and immune activation

. Furthermore, GL9 promoted dendritic cell maturation, highlighting its potential as an adjuvant for mRNA vaccines. Thus, GLs, which enhance both transfection and immune activation, were incorporated into five-component LNPs to establish a promising platform for mRNA vaccines and therapeutics, offering an innovative strategy to optimize LNP-mediated mRNA delivery.