生物
转移RNA
核糖核酸
生物发生
病毒学
病毒
病毒复制
核糖核酸酶
核糖核酸酶P
遗传学
基因
作者
Eun Jin Choi,Wenzhe Wu,Ke Zhang,Inhan Lee,In-Hoo Kim,Yong Rok Lee,Xiaoyong Bao
标识
DOI:10.3389/fmolb.2020.609732
摘要
Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infection in young children. However, effective treatment against RSV is unavailable. tRNA-derived RNA fragments (tRFs) are a recently discovered family of non-coding RNAs. We made an early observation that RSV infection causes significant induction of tRFs, which are mainly derived from the 5 ’-end of mature tRNAs (tRF 5 ). However, their functions and biogenesis mechanism are not fully understood. Herein, we identified an enzyme responsible for the induction of a functional tRF5 derived from tRNA-Gln-CTG (tRF5-GlnCTG). We found that tRF5-GlnCTG promotes RSV replication and its induction, assessed by Northern blot and a new qRT-PCR-based method, is regulated by ribonuclease ELAC2. ELAC2-mediated tRF5 induction has never been reported. We also found that ELAC2 is associated with RSV N and NS1 proteins. Given the fact that tRF5-GlnCTG plays a role in RSV replication, the identification of ELAC2 being responsible for tRF5-GlnCTG induction could provide new insights into therapeutic strategy development against RSV infection.
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