适体
检出限
亚甲蓝
纳米技术
环丙沙星
电化学
组合化学
线性范围
选择性
电极
化学
色谱法
材料科学
生物
有机化学
光催化
物理化学
催化作用
生物化学
抗生素
遗传学
作者
Seyed Milad Taghdisi Heidarian,Ashraf Tavanaee Sani,Noor Mohammad Danesh,Mohammad Ramezani,Mona Alibolandi,Golara Gerayelou,Khalil Abnous,Seyed Mohammad Taghdisi
标识
DOI:10.1016/j.snb.2021.129632
摘要
Abstract This study develops a novel electrochemical detection strategy for determination of fluoroquinolones (FQs) which was formed with a double-labelled aptamer as the detection element, methylene blue (MB) as redox agent and complementary strands of aptamer (CSs). This method exhibited excellent sensitivity for detection of ciprofloxacin as a member of FQs due to its ability to outstrip CSs lying flat on the electrode surface as a major challenge. Simplicity is another characteristic of this approach. The dynamic detection range was from 300 pM to 450 nM and the detection limit was 100 pM for ciprofloxacin. Also, the system displayed high selectivity towards FQs. The aptasensor succeeded in detection of ciprofloxacin in the spiked real samples, including human serum, milk and water samples. Altogether, the designed approach can introduce an effective and functional foundation for small molecules analysis to ensure food and human safety by applying corresponding aptamer sequences.
科研通智能强力驱动
Strongly Powered by AbleSci AI