免疫系统
纳米载体
抗原
免疫疗法
光动力疗法
癌症免疫疗法
材料科学
交叉展示
抗原呈递
癌症研究
卵清蛋白
免疫学
T细胞
医学
药物输送
化学
纳米技术
有机化学
作者
Huaiji Wang,Kun Wang,Lianghua He,Ying Liu,Haiqing Dong,Yongyong Li
出处
期刊:Biomaterials
[Elsevier]
日期:2020-06-01
卷期号:244: 119964-119964
被引量:64
标识
DOI:10.1016/j.biomaterials.2020.119964
摘要
Despite of the documented immunogenic cell death (ICD) and antigen cross-presentation (AC) in photodynamic therapy (PDT), the overall immune efficacy is rather limited. This study aims to expand the immune potential of PDT by spatially packaging antigen as photosensitiser nanocarrier to trigger efficient immune cascade for photodynamic immunotherapy. The package of ovalbumin antigen (OVA) into sub-100 nm nano-assembly is realized by driving intermolecular disulfide network between OVA molecules. OVA nanoparticles loading photosensitiser Ce6 (ON) are subsequently coated with B16-OVA cancer cell membrane, resulting in membrane cloaked ON (MON). Importantly, laser irradiation generated ROS significantly potentiates OVA antigen cross-presentation efficiency. Whilst, MON is endowed with homophilic targeting towards tumor due to cancer cell membrane coating. In treating B16-OVA tumor-bearing mice, MON effectively triggers the immune cascade, completely eliminates the tumor under laser irradiation and provokes a long-term antitumor immune memory effect. Conversely, a marginal effect is found if substituting OVA for bovine serum protein (BSA) in nanoparticle design or using MON to treat non-OVA expressing tumor. The antigen nanocarrier design promises to complement conventional PDT by boosting immune cascade, thereby leading to unique photodynamic immunotherapy.
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