粪肠球菌
次氯酸钠
光动力疗法
牙本质小管
共焦激光扫描显微镜
共焦
牙科
离体
生物医学工程
光敏剂
根管
牙髓治疗
材料科学
化学
体内
牙本质
医学
生物
光学
有机化学
大肠杆菌
生物技术
物理
基因
生物化学
作者
Roberta De Godoy,Carlos Eduardo da Silveira Bueno,Alexandre Sigrist De Martin,Rina Andréa Pelegrine,Carlos Eduardo Fontana,Marco Antônio Húngaro Duarte,Rodrigo Ricci Vivan,Wayne Martins Nascimento,Ana Grasiela da Silva Limoeiro,Daniel Guimarães Pedro Rocha
标识
DOI:10.1016/j.joen.2023.05.007
摘要
Introduction The aim of the present study was to investigate ex vivo by confocal laser scanning microscopy the antibacterial effect of photodynamic therapy (PDT) on dentinal tubules in the apical 5 mm of human mandibular premolars contaminated with Enterococcus faecalis. Methods Thirty-four teeth were standardized to 20 mm and foraminal anatomic diameters using a #20 K-file (Dentsply Maillefer). Samples were contaminated for 21 days and divided into the following 3 experimental groups (n = 10): the PDT group (instrumented canals and PDT), the passive ultrasonic irrigation (PUI) group (instrumented canals and PUI), and the PUI-PDT group (instrumented canals, PUI, and PDT), along with a control group (n = 4) (noninstrumented canals). The canals in the experimental groups were instrumented with ProTaper Next (Dentsply Maillefer) up to X3 and rinsed with EDTA and sodium hypochlorite. The photosensitizer used was 0.01% methylene blue with a preirradiation time of 5 minutes and a diode laser with 4 J energy and a 660-nm wavelength. Cross sections were made 5 mm from the apex of all samples, which were analyzed using confocal laser scanning microscopy. The results were analyzed using the Shapiro-Wilk and Kruskal-Wallis (Dunn) tests. Results There was a lower percentage of live bacteria in the PUI-PDT group, with a statistical difference compared with the control and PDT groups (P < .05). There was no statistical difference in the percentage of live bacteria between PUI-PDT and PUI (P > .05). Conclusions It was concluded that the PUI-PDT association was most effective in disinfecting root canals compared with the control group and PDT.
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