血清流行率
病毒学
寨卡病毒
人口
病毒
生物
抗体
医学
血清学
免疫学
环境卫生
作者
Shengyao Chen,Muhammad Saqib,Hafiz Sajid Khan,Yuan Bai,Usman Ali Ashfaq,Muhammad Mansoor,Abulimti Moming,Jing Liu,Min Zhou,Saifullah Khan Niazi,Qiaoli Wu,Awais-ur-Rahman Sial,Shuāng Táng,Muhammad Sarfraz,Aneela Javed,Sumreen Hayat,Mohsin Khurshid,Iahtasham Khan,Muhammad Ammar Athar,Zeeshan Taj,Bo Zhang,Fei Deng,Ali Zohaib,Shū Shěn
标识
DOI:10.1016/j.virs.2024.04.001
摘要
Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, the studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo haemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi virus (KSIV) based on a newly established luciferase immunoprecipitation system (LIPS) assays, and Zika virus (ZIKV) by enzyme-linked immunosorbent assays (ELISA). Neutralizing activities against these arboviruses were further examined from the antibody positive samples. The results showed that the seroprevalence of SFTSV, CCHFV, TAMV, KSIV, and ZIKV was 17.37%, 7.58%, 4.41%, 1.10%, and 6.48%, respectively, and neutralizing to SFTSV (1.79%), CCHFV (2.62%), and ZIKV (0.69%) were identified, as well as to the SFTSV-related Guertu virus (GTV, 0.83%). Risk factors associated with the incidence of exposure and levels of antibody response were analysed. Moreover, co-exposure to different arboviruses was demonstrated, as thirty-seven individuals were having antibodies to multiple viruses and thirteen showed neutralizing activity. Males, individuals aged ≤ 40 years, and outdoor workers had high risk of exposure to arboviruses. All these results reveal the substantial risks of infection with arboviruses in Pakistan, and indicate the threat from co-exposure to multiple arboviruses. The findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.