Dual-chamber up-flow fluidized hollow-fiber anaerobic membrane bioreactor (UF-HF-AnMBR) treating synthetic purified terephthalic acid wastewater: Granular sludge regranulation and methane production

Up-flow anaerobic sludge blanket is a representative way to treat high-strength industrial wastewater, but suffers from granule collapse and poor long-term performance. In this study, a dual-chamber up-flow fluidized hollow-fiber anaerobic membrane bioreactor (UF-HF-AnMBR) was constructed to enhance sludge regranulation and process stability for purified terephthalic acid (PTA) wastewater treatment. A stable and satisfactory aromatic compound removal (>99 %) was achieved at the organic loading rate of 7.15 ± 0.21 g-COD·Lreactor−1·d−1, with the methane yield of 0.345 ± 0.028 L·g-CODremoved−1. Although the granular sludge collapsed initially induced the pore blocking and membrane fouling, membrane separation promoted the agglomeration of small particles, sludge regranulation and biomass retention. Parallel factor analysis identified that the amino acid tryptophan in extracellular polymeric substances exerted a more critical effect on membrane fouling. The 16S rRNA and network analysis revealed the robust proliferation/enrichment of Methanosaeta, Pelotomaculum, etc., as well as the establishment of mutualistic symbiotic relationship among different species, ensured a stable microbial community and created a favorable circumstance for highly effective anaerobic treatment of PTA wastewater. This study proves that UF-HF-AnMBR can be an efficient and promising technique for sludge regranulation and microbe enrichment, advancing its application in PTA wastewater treatment and bioenergy recovery.