化学
化学发光
过氧化物酶
草甘膦
联苯胺
螯合作用
纳米传感器
辣根过氧化物酶
比色法
核化学
色谱法
生物化学
无机化学
酶
纳米技术
材料科学
农学
生物
作者
Ke Deng,Haixia Hu,Li Yi,Xue Li,Huiling Deng,Chen Ya,Xiao Yang,Lijun Wang,Xianggui Chen
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-06-01
卷期号:443: 138501-138501
被引量:1
标识
DOI:10.1016/j.foodchem.2024.138501
摘要
In this study, a dual-mode colorimetric/CL nanosensor was developed for glyphosate detection based on the specific inhibition of Fe3O4@Cu peroxidase-like activity. Synthesized Fe3O4@Cu exhibited high levels of peroxidase-like activity that triggered the oxidation of luminol/3,3′,5,5′-tetramethyl benzidine dihydrochloride (TMB) to excited-state 3-aminophthalic acid/blue oxTMB, thereby delivering a CL signal/visible colorimetric signal, however, the presence of glyphosate inhibited this activity, resulting in a decrease in signal strength. In-depth investigation revealed that this inhibitory mechanism occurs via two pathways: one in which glyphosates chelate with Fe(III)/Cu(II) and occupy the catalytical active sites of Fe3O4@Cu, thereby decreasing the generation of OH, and another in which glyphosate competes with TMB to consume generated OH, thus reducing the oxidation of TMB. This mechanism formed the basis of our novel dual-mode colorimetric/CL glyphosate nanosensor, which achieved limits of detection (LODs) of 0.086 µg/mL and 0.019 µg/mL in tests, thus demonstrating its significant potential for on-site glyphosate monitoring.
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