类风湿性关节炎
关节炎
滑膜炎
基质金属蛋白酶
肿瘤坏死因子α
炎症
医学
促炎细胞因子
免疫学
癌症研究
车站3
细胞凋亡
化学
内科学
生物化学
作者
Xiaoqin Zhong,Wei Feng,Lianjie Liu,Qingping Liu,Qiang Xu,Minying Liu,Xiaobao Liu,Shudi Xu,Minzhen Deng,Changsong Lin
标识
DOI:10.1016/j.intimp.2024.111487
摘要
Rheumatoid arthritis (RA) is an autoimmune disease that affects joints, causing inflammation, synovitis, and erosion of cartilage and bone. Periplogenin is an active ingredient in the anti-rheumatic and anti-inflammatory herb, cortex periplocae. We conducted a study using a CIA model and an in vitro model of fibroblast-like synoviocytes (FLS) induced by Tumor Necrosis Factor-alpha (TNF-α) stimulation. We evaluated cell activity, proliferation, and migration using the CCK8 test, EDU kit, and transwell assays, as well as network pharmacokinetic analysis of periplogenin targets and RA-related effects. Furthermore, we measured inflammatory factors and matrix metalloproteinases (MMPs) expression using ELISA and qRT-PCR assays. We also evaluated joint destruction using HE and Safranin O-Fast Green Staining and examined the changes in the JAK2/3-STAT3 pathway using western blot. The results indicated that periplogenin can effectively inhibit the secretion of inflammatory factors, suppress the JAK2/3-STAT3 pathway, and impede the proliferation and migration of RA FLS. Thus, periplogenin alleviated the Synovial inflammatory infiltration of RA.
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