胃蛋白酶
噬菌体展示
平移(音频)
重组DNA
肽库
生物
分子生物学
计算生物学
酶
生物化学
化学
肽
基因
肽序列
古生物学
镜头(地质)
缩放
作者
Shenglan Zhang,Jieyao Ma,Lin He,Qianying Li,Pan He,Jingyuan Li,Huicong Zhang
标识
DOI:10.1016/j.pep.2024.106431
摘要
Human pepsinogens (mainly pepsinogen I and pepsinogen II) are the major inactive precursor forms of the digestive enzyme pepsin which play a crucial role in protein digestion. The levels and ratios of human pepsinogens have demonstrated potential as diagnostic biomarkers for gastrointestinal diseases, particularly gastric cancer. Nanobodies are promising tools for the treatment and diagnosis of diseases, owing to their unique recognition properties. In this study, recombinant human pepsinogens proteins were expressed and purified as immunized antigens. We constructed a VHH phage library and identified several nanobodies via phage display bio-panning. We determined the binding potency and cross-reactivity of these nanobodies. Our study provides technical support for developing immunodiagnostic reagents targeting human pepsinogens.
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