CeMn-based nanozyme as an ‘Electron Pump’ to significantly enhanced antioxidant for Intestine-Reperfusion injury

Chasing redox nanozymes with recover cellular homeostasis provide potentials towards intestine reperfusion injury, but remained a challenge goal. Herein, for the first time, we constructed an efficient redox nanozyme by constructing enzyme mimicking active center and investigated its formation and enzyme-like mechanism thoroughly. Specifically, the partial Mn substitution in CeO2 (donated as CeMn-zyme) demonstrated to alter electronic structure with abundant defect site generation, including Mn substitution and oxygen vacancy (Ov) defects which significantly enable the SOD-like and CAT-like activity of CeMn-zyme in a synergetic manner, which can protect intestinal cells from intestine reperfusion injury, further recovering cellular homeostasis. More intriguingly, both Density Functional Theory (DFT) calculation and transcriptome data can further identify the remarkable enzyme-like activity and the specific factor alternations. Taken together, this CeMn-zyme has promising application potential for intestine-reperfusion injury, paving the way for the clinical translation from bench to bedside.