Recent Progress in Mass Spectrometry‐Based Single‐Cell Metabolic Analysis

Abstract Single‐cell analysis enables the measurement of biomolecules at the level of individual cells, facilitating in‐depth investigations into cellular heterogeneity and precise interpretation of the related biological mechanisms. Among these biomolecules, cellular metabolites exhibit remarkable sensitivity to environmental and biochemical changes, unveiling a hidden world underlying cellular heterogeneity and allowing for the determination of cell physiological states. However, the metabolic analysis of single cells is challenging due to the extremely low concentrations, substantial content variations, and rapid turnover rates of cellular metabolites. Mass spectrometry (MS), characterized by its high sensitivity, wide dynamic range, and excellent selectivity, is employed in single‐cell metabolic analysis. This review focuses on recent advances and applications of MS‐based single‐cell metabolic analysis, encompassing three key steps of single‐cell isolation, detection, and application. It is anticipated that MS will bring profound implications in biomedical practices, serving as advanced tools to depict the single‐cell metabolic landscape.