噬菌体
生物传感器
纳米技术
等离子体子
胶体金
纳米颗粒
材料科学
光电子学
生物
遗传学
基因
大肠杆菌
作者
Joung‐Il Moon,Eun Jung Choi,Younju Joung,Jin Woo Oh,Sang‐Woo Joo,Jaebum Choo
标识
DOI:10.1016/j.snb.2023.134916
摘要
In this study, a novel nanoplasmonic substrate is developed for biomedical applications. In nanoplasmonic biosensors, a substrate that can induce strong hot spots by forming uniform nanogaps smaller than 10 nm is required to implement a device capable of detecting biomarkers at low concentrations. Herein, gold nanoparticles-encoded M13 bacteriophage (AuNPs@M13) substrate was developed by immobilizing 60 nm AuNPs onto the surface of an M13 bacteriophage scaffold with a network assembly structure. The plasmonic characteristics of the proposed AuNPs-M13 were evaluated by assessing the nanogap distribution, electromagnetic enhancement effects, and reproducibility of the fabricated substrate. Additionally, an immunoassay for severe acute respiratory syndrome coronavirus 2 was performed to confirm the clinical applicability of the proposed AuNPs@M13 substrate. When the AuNPs@M13 was used as a nanoplasmonic substrate, it was possible to achieve better sensitivity (limit of detection [LoD] = 1.21 PFU/mL) than that with the commercially available lateral flow assay strip (LoD = 300–500 PFU/mL) or enzyme-linked immunoassay (LoD = 48 PFU/mL). The proposed AuNPs@M13 in this study is expected to be used as an innovative biomedical device for the high-sensitivity detection of various biomarkers.
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